Centrifuges
Due to the high speed at which they operate, centrifuges have great potential for injuring users if not operated properly.
Unbalanced centrifuge rotors can result in injury, even death. Sample container breakage can generate aerosols that may be harmful if inhaled.
The majority of all centrifuge accidents are the result of user error. To avoid injury, workers should follow the manufacturer's operating instructions for each make and model of centrifuge they use.
Follow these steps for the safe operation of centrifuges:
- Ensure centrifuge bowls and tubes are dry.
- Ensure the spindle is clean.
- Use matched sets of tubes, buckets and other equipment.
- Always use safety centrifuge cups to contain potential spills and prevent aerosols.
- Inspect tubes or containers for cracks or flaws before using them.
- Avoid overfilling tubes or other containers (e.g., in fixed angle rotors, centrifugal force may drive the solution up the side of the tube or container wall).
- Ensure the rotor is properly seated on the drive shaft.
- Make sure tubes or containers are properly balanced in the rotor.
- Only check O-rings on the rotor if you are properly trained.
- Apply vacuum grease in accord with the manufacturer's guidelines.
- Do not exceed the rotor's maximum run speed.
- Close the centrifuge lid during operation.
- Make sure the centrifuge is operating normally before leaving the area.
- Make sure the rotor has come to a complete stop before opening the lid.
Employers should instruct workers when centrifuging infectious materials that they should wait 10 minutes after the centrifuge rotor has stopped before opening the lid. Workers should also be trained to use appropriate decontamination and cleanup procedures for the materials being centrifuged if a spill occurs and to report all accidents to their supervisor immediately.
Knowledge Check Choose the best answer for the question.
9-2. Which of the following physical hazards can cause laboratory worker injury or even death?
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